KMID : 0624620190520080526
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BMB Reports 2019 Volume.52 No. 8 p.526 ~ p.531
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Akap12beta supports asymmetric heart development via modulating the Kupffer¡¯s vesicle formation in zebrafish
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Kim Jeong-Gyun
Kim Hyun-Ho Bae Sung-Jin
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Abstract
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The vertebrate body plan is accomplished by left-right asymmetric organ development and the heart is a representative asymmetric internal organ which jogs to the left-side. Kupffer's vesicle (KV) is a spherical left-right organizer during zebrafish embryogenesis and is derived from a cluster of dorsal forerunner cells (DFCs). Cadherin1 is required for collective migration of a DFC cluster and failure of DFC collective migration by Cadherin1 decrement causes KV malformation which results in defective heart laterality. Recently, loss of function mutation of A-kinase anchoring protein 12 (AKAP12) is reported as a high-risk gene in congenital heart disease patients. In this study, we demonstrated the role of akap12¥â in asymmetric heart development. The akap12¥â, one of the akap12 isoforms, was expressed in DFCs which give rise to KV and akap12¥â-deficient zebrafish embryos showed defective heart laterality due to the fragmentation of DFC clusters which resulted in KV malformation. DFC-specific loss of akap12¥â also led to defective heart laterality as a consequence of the failure of collective migration by cadherin1 reduction. Exogenous akap12¥â mRNA not only restored the defective heart laterality but also increased cadherin1 expression in akap12¥â morphant zebrafish embryos. Taken together, these findings provide the first experimental evidence that akap12¥â regulates heart laterality via cadherin1.
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KEYWORD
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AKAP12, Asymmetric development, Heart laterality, Kupffer¡¯s vesicle (KV), Zebrafish
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